Abstract:
The objective of present study was to determine the inhibition capacity and modes of inhibition of
fresh and debittered Vateria copallifera seed extract against alpha-amylase and alpha-glucosidase
enzymatic reactions. In vitro assessment of alpha-amylase and alpha-glucosidase enzyme inhibition
capacity was evaluated for 80% ethanol, 80% methanol and aqueous extract of fresh and debittered
V. copallifera seeds. Modes of inhibition (Kinetic analysis) were identified using Michaelis Menten
and Lineweaver-Burk (double-reciprocal) plots. Results revealed that, 80% ethanol extracts showed
the significantly highest alpha-amylase enzyme inhibition capacity (fresh IC50: 36.97±1.28
𝜇g/mL, debittered IC50:98.58±0.55 𝜇g/mL) followed by aqueous extract (fresh IC50: 258.14±12.41
𝜇g/mL, debittered IC50: 410.44±1.24 𝜇g/mL) compared to positive control Acarbose. The highest
alpha-glucosidase enzyme inhibition capacity is given by 80% ethanol extract of fresh V. copallifera
seeds (IC50: 1469.10±10.01 𝜇g/mL). Kinetic analysis revealed that, 80% ethanol extract inhibited
the alpha-amylase competitively although extract displayed a non-competitive mode of inhibition
towards alpha-glucosidase. Thus, Vateria copallifera seeds can be considered a good natural
resource for the management of Type 2 diabetes with postprandial hyperglycemia due to their
traditional acceptability as a healthy food ingredient, availability and low costs with the necessity
of further investigations on its active components.
